![]() ![]() When germination occurred at supraoptimal temperatures (30 – 35 ☌), DAO activity was sharply inhibited both in the cotyledons and in the embryonic axis. ![]() The absence of cotyledons promoted the early appearance of DAO in the embryonic axis. The highest DAO activity in the embryonic axis of seedlings grown for 72 and 96 h was found in the elongation, differentiation and hypocotyl zones, while the lowest was in the apex and plumule. ![]() From the time when the embryonic axis acquired an aerobic metabolism, DAO increased concomitantly with the growth of the embryonic axis and at the same time with the deterioration of the cotyledons, although in these organs the values were clearly lower than in the axis. Diamine oxidase (DAO, EC 1.4.3.6.), which participates in oxidative catabolism of polyamines (PAs), was not detected in the dry viable chickpea (Cicer arietinum L.) seeds. ![]()
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